FastQCFastQC Report
Fri 10 Jun 2016
402629_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402629_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887074
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAA450.001814643473.689561
CTTAAAT701.3185678E-463.162472
TATAAGA600.005673355455.2671662
TCCTATA650.007786102651.0158422
GTCCTAA904.5634102E-449.126371
ATACAGT1150.001525460938.4467245
GGTATAT1951.4801533E-534.0105631
ATACTCA1753.1270384E-431.581241
GTTAGAG1400.004003055431.5812363
GTATGTT1400.004003055431.5812361
CTAAGTG1550.00658439428.5249883
CTACACT1650.00893336426.7962024
CTGTATT2350.001750572723.5179429
ATAGGAC2902.2095504E-422.8691733
GTACTGT2500.002508321322.1068671
TAGGACT2500.002508321322.1068674
CGTAATA2550.002813895221.6733992
TGTAGGA3103.4689912E-421.3937422
TACTACA3153.8648577E-421.054162
TATGCCT3204.298315E-420.7251874