FastQCFastQC Report
Fri 10 Jun 2016
402267_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402267_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684273
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATT853.5562352E-667.404514
TTTTTAA2350.002106074736.00281235
TATATGA1601.4958334E-435.8086432
CTAAATA1300.002334210635.2577443
TAAGGGT2951.7777529E-731.074624
TATAGGG2300.001252748924.910362
CTATAGG2851.5387306E-424.125151
TGTATAC2951.944796E-423.3059643
GTGTAGA10900.021.0265061
GTGTTTA3854.8920898E-520.8353581
ATTAGGC2800.00392889620.4620823
ATTATGC2850.004352859720.1030983
CTACACT3455.6019734E-419.928294
TGTAGTA4659.348758E-619.7140052
TAATCTG2950.005313793719.4216375
GTCTTAG3658.1824185E-418.8374481
AATAGGG4301.1446157E-418.6538052
ATAACAC3708.969473E-418.5817833
CTCTACA6202.9066723E-718.4818782
TGTAGAA7606.8230293E-918.0927892