FastQCFastQC Report
Fri 10 Jun 2016
402267_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402267_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684273
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACA1255.4387783E-754.987562
TTAGTAC700.00906201649.0960353
CTTACAT2005.808033E-945.8229683
TATAGGA1406.824771E-540.9133642
CTCTATA1951.1593213E-535.2484362
TCTTACA1802.99221E-431.8215072
TTATAGC1853.5137247E-430.9614622
TAGTATG1500.004711323430.5486474
CTTACAC1904.1080825E-430.146693
CTTAAGT1550.005530582729.5632043
TAAACTT1550.005530582729.5632045
AGATTTA1550.005530582729.5632046
TAGGACT3902.1354936E-929.3736974
GTACACT1650.007505574327.7714981
ACATAAT1650.007505574327.7714981
ATAGAGT1700.00868230626.9546854
GTAATAA1700.00868230626.9546851
GTATTAC4201.4185207E-724.5480181
TTAATGG2350.001422177524.3739193
TTATACA2350.001422177524.3739194