FastQCFastQC Report
Fri 10 Jun 2016
402138_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402138_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762065
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAG600.00507161456.8546684
TGTATAG1200.00163586137.9031142
GATGTAA1651.8743976E-434.457381
GTCTGTA2056.690059E-427.7339862
GGTAATA1700.00899963226.7551381
GTGTAGA4701.6858394E-824.1934781
GTAAAGG2550.002386962822.2959484
TCATAGA2650.00298415121.4545942
GGAGGTT6053.579347E-421.290676235
TAGAACC2800.004105882320.3052394
AGGGTAT2800.004105882320.3052396
GTAGTGA2800.004105882320.3052396
TAACACC4057.6599004E-519.6534674
TATTGAT3000.00611887218.9515572
GTATACG3000.00611887218.9515571
TCTTGTA3100.007393627418.3402162
TAGGTCC3150.00810816718.04914
CTAAGCC3150.00810816718.04913
AGGAGTG3850.001232378917.7209365
TAGCTGT4000.00159203617.05644