FastQCFastQC Report
Fri 10 Jun 2016
402138_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename402138_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762065
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGA355.991326E-497.4042
CTAATAC802.2250373E-456.8189933
TACATAG700.00935322548.7022
CCCTTAA700.00935322548.7021
CATATAC1058.509222E-443.2906653
CTTAGTC1100.001069839641.3229063
TGTACTA1702.2419218E-433.422942
CTTTAGG1500.00489904430.3034692
TATGGAG1650.007803963527.5486055
GTATAGC2608.725165E-526.2241541
ACGTGTA2901.8312718E-423.511317
ATATACC2450.001898653623.1914274
TAGACCC2952.0561554E-423.1128124
CTTCACA2500.00213529822.72761
GTATTGA3552.788796E-522.4074921
CATACCT3203.5647364E-421.3071234
TACGGAG2850.00456536919.936497
GTACAAG3557.1767246E-419.206421
ACACACT3000.006141087518.9396694
AGAAAGG3607.8846357E-418.9396675