FastQCFastQC Report
Fri 10 Jun 2016
401391_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename401391_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191995
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATTA604.947173E-576.991441
TATTAAT500.002317568369.283582
TCATATA1152.8928844E-760.2465862
TATGTTA650.006550899753.295062
TAATTAC1101.5662965E-552.487562
TTAGGAG1006.1852665E-446.1890533
TATAGGA1355.2538628E-542.767642
ATGTTAA1200.001516154238.495721
CTCTATA1200.001517092138.4908752
ATGTTAC1250.001852782736.9558941
ATATTAC1300.002246098635.534511
TAAGACA1651.7129062E-434.9917074
TTAATGT1400.00323218232.9921764
TAGGAGT2702.9468029E-629.9373474
CTTAATG1550.00531897929.7993892
TTTATTC1550.00531897929.7993893
TGTAAAC1954.5645292E-429.6083682
TATTACG4103.3451215E-928.1640552
TAGATTG2056.11644E-428.1640555
GTGTTAG3304.4291846E-727.996891