FastQCFastQC Report
Fri 10 Jun 2016
401391_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename401391_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191995
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAT500.002318272869.278276
TAGATAG1358.758743E-751.317235
ATATAGA1451.4334964E-647.778121
TCTTAGT1109.893705E-441.9868282
CTTATGT1458.010586E-539.8150981
TTAAAGG1200.001517663838.4879263
GTCTTAG3001.1823431E-1038.4879261
AATTATG1400.003233395732.989652
TTGTATA1450.00383969231.8520774
TGTATAG1500.00453263330.790345
TCTAGAG1550.00532097229.7971042
TATAGAC1550.00532097229.7971042
GTCTAGT1954.566578E-429.6060981
GTATATG1954.566578E-429.6060981
GTATAGG3552.5731424E-829.2725051
TATAGGG2107.0449774E-427.4913772
TCTAGTC1750.00962225126.3917222
CTTAGTC1750.00962225126.3917223
TAGCACT3201.104681E-525.2577024
GTACTAC6051.2732926E-1124.8103961