FastQCFastQC Report
Fri 10 Jun 2016
400958_S3_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400958_S3_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054892
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATA500.002580799467.428444
GTATTAG700.00976536948.1745871
GTATAGA1250.00211556535.970361
GTGTATA1601.674273E-435.1273041
GTCCTAA1400.003687242332.1163941
ATAAGAC1550.00607309329.0014783
TCCTAGT1600.007091645628.095182
TACAAAG2006.2041724E-428.095182
CTACAGA2558.236333E-526.4487951
TTTACAA1700.00953198726.4425243
TTATTGA2250.001234095124.9734944
CTGTTAG2300.001400783924.4363841
GTATGAG3351.9386976E-523.4881081
GAATAAG2400.001794623223.4182031
GTCTTAG2450.002023268522.940281
TACAGGA3452.438253E-522.801892
TATACCG5453.6907295E-922.6823485
GTATATC2500.002275350722.4814741
TAGGACT3103.1021246E-421.751114
ATTAGGG2600.00286120321.611683