FastQCFastQC Report
Fri 10 Jun 2016
400958_S3_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400958_S3_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054892
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA853.193163E-452.8136862
CTATTAA650.00733191951.7980421
TATAGGA650.00733191951.7980422
GTATAAG904.2337977E-449.8795931
CCTTTAA955.527475E-447.254351
CTTATAT1007.117051E-444.8916321
TCTATAC1501.1561493E-437.4096953
TACTACT2258.864645E-734.9157144
TATAAGG1300.002584452334.532032
TAAGACC2658.3180566E-833.8804784
AGTAGGA1350.003109662833.253062
TTACATG1350.003109662833.253062
TATATGG1400.00371601432.0654532
TAAGAAC2203.043775E-530.6079333
TTAACTC1550.006113289428.9623434
TGTACTA2006.253358E-428.057272
TATACGA1600.007138546628.057275
TACTACA3857.4487616E-826.235372
TAAGGGT2400.001811273323.3810584
TAGGAGA2901.9935242E-423.2198094