FastQCFastQC Report
Fri 10 Jun 2016
400739_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400739_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563153
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGT1400.003583289732.3062482
CTATAGT2200.001044904525.6981534
CTATATA2250.001190417125.1300241
ATACTTA2250.001191229925.1270836
CATAGTA2350.001534716124.0578442
TATAGTG2550.002467768322.1709545
GTATAAC3603.2298685E-521.988771
GTGTTAT6206.384653E-1021.8874381
CTTAAGA3153.3178847E-421.5374982
GTGTAGA16050.021.1374031
CTTATAA3150.001524306420.671192235
TTAGTCC3304.5421842E-420.5585234
GTATTAC6251.6674676E-819.9029791
TAAGAAC5751.5141813E-719.6646733
GTGTATG11750.018.2861021
GTGTAAA4951.7965349E-518.276381
TATACAG3750.001073573518.09155
TATAGGT3200.00917625217.667482
TTGTATT4602.1243449E-417.2065892
TAGTAGA4652.3072104E-417.0215722