FastQCFastQC Report
Fri 10 Jun 2016
400739_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400739_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2563153
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT1250.00206812436.138931
TATTTAG1601.6295313E-435.2919242
ATATAGA2952.0200605E-730.6262111
GTATTTA3002.340621E-730.1157761
TTACATA1904.4668838E-429.7195152
TATAGAC3254.7158574E-727.7991752
CTATAGA2250.001199723725.0964781
CCTTATA2350.001545645224.0285422
TCCTATA2450.001969771723.0477852
TGCTATA2450.001969771723.0477852
TAATACC4002.8792165E-622.5868324
TATACAG3502.6253792E-522.586835
TATTACA2550.002485295522.1439512
TTAAGCA4103.5673056E-622.035933
TTATACA3203.720814E-421.1751544
TTAAGGT3203.720814E-421.1751544
TATACGA4907.194503E-720.7430082
CCTTACA2850.00473545319.813012
TGTAGCA8154.7293724E-1119.399732
TATATGA3557.489644E-419.0874615