FastQCFastQC Report
Fri 10 Jun 2016
400541_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400541_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005601
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGGG954.7998023E-448.6265034
GTCTAAT1250.001851679236.9598161
TTCCTAA1300.002244761735.5382841
TTTATGT1300.002245857635.534753
TGTAACA1651.7113812E-434.996354
CCTTAGG1702.0395288E-433.967042
ATATAGA1752.4165964E-432.9998361
CCTAACT1752.4180081E-432.996552
TAACTGA1752.4180081E-432.996554
TTCTATG2303.3909808E-530.1272892
CTTTATG2107.035595E-427.497132
GTGTTAA1700.00834077927.1763361
ATTTAGC2300.001196546525.1060753
TAGGACT2500.001944384723.0975884
ATAGAAC4153.2607804E-622.2627353
GTATATC2600.00244074222.2114281
CTCTGAG2600.002442152522.209222
TATCACA2650.002727742821.7901784
ATACACG3455.3121476E-420.084866
TCTATGT2900.004599339319.9117158