FastQCFastQC Report
Fri 10 Jun 2016
400541_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename400541_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005601
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAG550.003383985762.972374
TATAACA600.004776182657.7246743
TATATAT903.6821183E-451.310822
ATAGTAC700.00878749149.4782943
GTCCTAA1006.1909517E-446.1797371
AAACGTA2506.89324E-540.83834235
TATACAG1500.004535072530.7864915
CTCTTAC1903.9244757E-430.381411
TTAGGGA1550.00532383229.7933792
TTCTAGG2353.936008E-529.4764292
GTATAGC3754.3859472E-827.7078441
TATGGAG1700.00835844227.1645532
GCATATA1700.00835844227.1645531
ATAAAGA1750.00962741226.3884222
TATATCC2209.2503236E-426.2384873
TATACCC3052.3159162E-422.7113463
GTATATC2550.002185858322.6371271
GTGTTAG2600.002446897622.2017971
GTACTGT3703.398816E-521.841776
CCCTATG4304.4494427E-621.4789492