FastQCFastQC Report
Fri 10 Jun 2016
301747_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename301747_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363465
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTA550.00356157162.165792
TGTAATA1200.001617968837.9902082
CCTAGTA1300.002396702535.0678832
ATACACT1400.003446447432.5630344
CAATTAG1500.00482737430.3966261
TATATCT1500.00483083930.3921664
CCTAGAA1904.2294888E-429.9966721
TATAGAC1550.005670776629.4117742
ATATTTG3050.007619960227.683516235
TACACTG2659.741691E-525.8046685
CTAGACC5501.2914825E-1024.8663184
CCTAAGA2300.001292671324.7762222
TTAGTGA2300.001292671324.7762223
TAGTACA2300.001292671324.7762224
CGTAAGA2400.001656285723.743882
TAAGGAC2500.002100173822.7941254
ACCTAGA3052.5277003E-422.420452
CTTACAC2550.002356296222.3471813
CTAGAAG2600.002637563321.9174272
GCTTACA2650.002945860621.5038912