FastQCFastQC Report
Fri 10 Jun 2016
301747_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename301747_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363465
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAA1150.005795005754.97068235
TTAGAAG955.135163E-447.9662782
TATAGAG1458.6724525E-539.282732
GTCTATA1601.5479795E-435.5999761
TATAACA1400.003453967932.5485464
GTTTAGT1803.091463E-431.6444231
GACTAAT1450.00410142531.4261841
CTAGTAC1500.00484136530.3786453
GTGTAAT1904.2441746E-429.9789241
CCTATAG1550.005683121729.398693
GTCTTAG2200.001000089228.734673235
TAACACA1600.00663659828.479984
ATATAGA1650.007712276727.616951
CTATGCT2300.001296028124.7651984
GTATAAC2450.001872241523.2489641
CCTAGGA3002.2669628E-422.7839832
GTATACA2650.002953474621.4943261
ATTGGAG5903.429583E-421.429249235
TGATAGT3754.1792922E-521.265054
TGTAGGA6051.053013E-820.7127132