FastQCFastQC Report
Fri 10 Jun 2016
300234_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename300234_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991246
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTA1300.00918689248.921715235
CCCTAGT2001.3485109E-534.4769821
TATAGGC2707.971721E-834.044472
TATATCA1802.9393754E-431.916694
ATCTAGA1450.003928592431.7029741
TCTAACA1450.00393247631.6965772
ATTAGGC2551.9611489E-631.5412033
CTATAGG1600.00635754628.7308181
CATATAT1650.007395511527.8545682
GTCTTAT1700.00854666527.040771
TCCTACT2158.31668E-426.7209512
GTATTGA4751.686385E-824.1943761
TCCTGTA3351.6359043E-524.0089742
GTCCTAT2901.6973459E-423.7772311
CTAGAAC2500.002002926822.9800193
GTACAGA3602.8495437E-522.3461911
GTATAGG2700.003127866721.2820871
GCTAGAA2750.003483033720.8909262
ATAAGCC2800.00386631620.5178743
GTATACG4006.4188105E-520.1115721