FastQCFastQC Report
Fri 10 Jun 2016
300234_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename300234_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991246
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAC500.002373823268.864933
CCATTTA1150.006545349553.30273235
CAGTATA650.006709491452.9730262
ATATTTA700.00899327849.1892361
AATAGAG954.9486273E-448.3262672
TATAGAA1006.3722633E-445.9099542
CTTATAG1058.1034016E-443.7237663
CAATAGA1704.462072E-640.508781
TTATAGC1300.002315132435.315354
GTATAGA1300.002315132435.315351
TCTAGGA1702.1150695E-433.7573172
CCTTTAT1450.0039535231.6620351
CTACACT1450.0039535231.6620354
CTTACAC1450.0039535231.6620353
TAGGACT2652.6699163E-630.3178924
GTCTTAG1954.728855E-429.4294591
TCCTACA2506.2454565E-527.545972
ACTGTAC2107.2948466E-427.3273538
TTCTATT1750.00990623326.234262
TACATAC1750.00990623326.234263