FastQCFastQC Report
Fri 10 Jun 2016
16885_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16885_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001983
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAG1401.1383399E-649.4021871
CTATAAG1057.932888E-443.913061
GTATATG1406.577252E-541.168491
TCTAAGA1450.003871757331.7975232
GTCCTAT3301.2845703E-831.4377561
TCCTATG3401.7185812E-830.5115932
GTATAGG2652.5815334E-630.4491481
TACATAG1600.006265770628.8165052
GTTTTTT3958.5027236E-426.617315235
ATGTGTA2851.478346E-424.2665312
TAGAAGA2650.002758358821.7483064
TATGACA4254.076215E-621.6971344
TACATGA3253.5967666E-421.279882
TATAGGC2800.003795784720.583222
TAATTGC2850.00420547920.2221094
CTAACCA2900.00465079619.8734514
ATATGAC4107.585225E-519.6795653
TACAAGG4158.325595E-519.4424612
TAACACC5501.7879138E-618.8617134
TACTGAG3250.00897830617.7332342