FastQCFastQC Report
Fri 10 Jun 2016
16885_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16885_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001983
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA600.00481294757.612951
CCCTATA1355.319975E-542.6762582
TCTAACA1150.001243633740.078572
ATATAGA1400.003265984632.9216841
TATAAGC1450.003878363931.7864533
TCTAACC1600.006276437528.8064732
GTTAGAC2056.1927387E-428.1038763
TTACTAC2506.080917E-527.6542153
TTAGGGT2107.1296096E-427.4347364
ATAGCAT1750.00971873726.3373473
TAAACCT1750.00971873726.3373474
ATATTCT2250.001066638225.6057534
CACTAGA2951.8722212E-423.4357742
CTAACTC2500.001970234623.045184
GTTCTAT3152.9188243E-421.947791
GTAGAGA3505.9431774E-419.753013
TGTTACG3505.9431774E-419.753012
TAGGACG3505.9431774E-419.753014
TCATACA4251.0022321E-418.9783842
TCTGATA3150.00751349418.2898252