FastQCFastQC Report
Fri 10 Jun 2016
02-05_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename02-05_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822205
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG700.00857736649.7814371
TTATAGA1406.2862615E-541.484534
ATATAAA1406.2862615E-541.484531
CTATAGC1200.001473426438.7188953
TCTTATA1651.6540084E-435.1992
GTTAAGA1350.002626824734.4167981
GTCTAAT1400.003139420933.1876261
GTCTTAT1752.3370466E-433.1876261
GTACATA1450.003728177232.0432241
TCCTATG2252.8049464E-530.9751192
AAATAGT1550.005166659629.9759225
TCCTGTA2854.285708E-628.5297152
CTTATAG1650.007012398428.15923
GTGTAAC1650.007012398428.15921
ATATACC2106.801047E-427.6563554
GTCCTAT1700.00811221727.3309861
TGTTGGG5104.0017767E-1127.3309862
CTAGAGG3050.00869464826.942795235
GTATTAC2607.5170676E-526.8053911
TAAGGAC2658.557095E-526.299633