FastQCFastQC Report
Fri 10 Jun 2016
02-05_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename02-05_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822205
Sequences flagged as poor quality0
Sequence length32-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAG500.002266423569.672621
TAATATG550.0033068863.338743
GTACTAG600.004667449358.0605131
GTATAGG1203.7333848E-758.0605131
TGTTAGA1253.2260257E-546.4484062
GTATAAG1109.6194295E-442.2258261
TAATAGA1406.297656E-541.4717944
ATATAGA1704.1176427E-640.9838941
TACTAGG1150.00119707440.3899232
TATTACA1457.7449746E-540.0417332
CCTATAC1601.3827268E-436.287823
GGATATA1450.003733787732.0333861
CTCTATA1450.003733787732.0333862
CTAGTAG1500.004407707630.9656071
ATTACAC2303.2660435E-530.292443
TATAAGG1550.00517442429.9667153
CCCTAGA1600.006042972729.0302561
GTCATTA1700.0081243827.3225961
TCTATAC1700.0081243827.3225963
TACAGAC1750.00935801126.541952