FastQCFastQC Report
Tue 15 Mar 2016
7-Wingbud11B2_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename7-Wingbud11B2_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21541433
Sequences flagged as poor quality0
Sequence length10-100
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA33350.029.7398241
GCCCCGT26000.028.0993861
CCCCCGT26150.025.9063341
CCCCGTA30200.023.7516772
CCCCGAT29100.023.1279342
CCCCGTT45800.023.0089972
CCCCGAA45850.022.2113382
GGCCCCT33000.022.138911
GGGGGTT55400.021.339671
GGGGGGT36700.020.7514571
GCCCGTA22650.020.7216262
CCCGAAT47800.020.3789123
GGGCCGT24950.020.053681
GGGCCCT26900.019.5875851
GCCCCCA35450.018.8602261
TCCCCGT46650.018.6033291
CCCGTTT82100.018.4445973
GCCCGGT27150.018.265622
GGGGGCA39050.018.0286141
TGCCCGT33900.018.02461