FastQCFastQC Report
Tue 15 Mar 2016
7-Wingbud11B2_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename7-Wingbud11B2_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296663
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT555.196267E-639.6066781
GTGAGCG857.606468E-834.2108577
TCGCTAA450.003103026233.25940390-91
TGAGCGC901.2511373E-732.3266338
GGACCAT702.6494987E-531.2248659
TAGCCAA702.7114067E-531.1195341
AGCGCGT503.0516094E-729.2099410-11
GAGCGCG1003.104451E-729.1432049
GGGTGTG1205.192669E-827.2158223
TGACCGG750.0014784224.204082
CGCGTTG750.001482783624.1918414
GCGTACC600.001746784823.5224968-69
CGCTAAT850.002568987222.00989790-91
GACCGTG1003.063711E-421.7836742
ACCGTGA1003.074211E-421.7726553
CGCGTGG705.5657583E-620.9281412-13
AGGGACC1054.2257376E-420.7708787
GGTGTGA1801.1563134E-720.1598684
CATCCGT550.004550546419.94597840-41
GCTGGGG1105.8288744E-419.8033391