FastQCFastQC Report
Tue 15 Mar 2016
60_Embryo23B_140204_L008_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename60_Embryo23B_140204_L008_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19637927
Sequences flagged as poor quality0
Sequence length10-100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232380.11833224555728311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA29100.030.8738331
GCCCCGT22150.025.8477671
GCCCCGA19800.025.3567181
CCTGACC52850.024.5827453
CCCCGTT45100.022.5573882
CTAGGCG39100.021.96612538-39
GACCCGA15100.021.8744771
GCCCGGT32100.021.814512
CCCCGAT32250.021.7130472
GGGCCGT18500.021.4251651
TAGGCGC41400.021.34161638-39
CCCCGAC22800.021.0545062
CCCGGTA38800.020.885243
GGGCCCT22650.020.8050581
ATAATCG77200.020.74826894
CCTAGGC42200.020.62678736-37
TCCCCGT36450.020.5402051
CCCCGTA25550.020.5120682
CCCGAAT48750.020.2360973
GGCGCTA42700.020.21292340-41