FastQCFastQC Report
Tue 15 Mar 2016
60_Embryo23B_140204_L008_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename60_Embryo23B_140204_L008_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187769
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA603.4015102E-1094.4774294
GACGGAA500.0094114148.58838794
AAATCGG705.7480065E-945.83112792-93
CACGGAC359.830581E-442.0230563
GCCTCGA400.001895117836.7701724
AACACGG450.003376739832.6845971
ACACGGA603.721581E-430.6418112
CGGACCA550.00893434426.7847425
GCCCATG709.1692223E-426.264413
CCACCAG951.9830471E-423.2232672
CGCGAGT500.00457343319.92668328-29
GGAGTCT500.00693243918.5431112-13
GTGTGCA1300.001591186917.048038
AGTGTGC1400.002620286315.8218497
TGTGTGG1550.00512110514.3060039