FastQCFastQC Report
Tue 15 Mar 2016
6-Wingbud11A_131203_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6-Wingbud11A_131203_L002_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24485287
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356920.14576917150287028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT30450.029.8805791
TCGGTCC73950.027.05289588-89
AGTCGGT76750.025.88429386-87
CTGAGAG127100.025.3373194
CGGTCCT77550.024.90867688-89
AAGGCCG76200.024.72434836-37
AGGCCGA77550.023.24149736-37
CCCCCGA49400.023.1800961
CCCCGAT31100.023.1194292
GTCGGTC85400.022.49931786-87
CCTGACC69100.022.3523733
CAGTCGG86100.021.95446884-85
GGGGGGT31650.021.4555761
CCCCCGT32500.021.4406911
CCCCGTA37250.021.0896552
GGGGGTT55350.021.0090371
GGCCCCT35900.020.893671
CCCCGTT59700.020.8164252
TCAGTCG100350.020.01738284-85
CCCCGAA56050.019.7964292