FastQCFastQC Report
Tue 15 Mar 2016
6-Wingbud11A_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename6-Wingbud11A_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367322
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCA501.539968E-435.622775
TGACCGG450.003988401531.6000254
CCCGCAG1050.00416257931.31441794
CCGGGGA1650.030.163664
GGACCGA500.006609669428.4982155
ACCGCAC754.9969778E-528.4555171
GCCGGGT905.451504E-627.6537863
GCTCCCG657.017569E-427.5034228
GGGCTCC657.1136036E-427.4395566
AACACGG657.233727E-427.3610731
CGGGGCT657.256796E-427.3461744
GCCGGGG1850.026.9063873
CGTCGGT659.071139E-426.3214182-83
GAGCGCG1409.296855E-925.5774129
GGCCTAT700.001117621125.3963323
GTGAGCG1751.546141E-1024.4871647
GACTAGC901.5445385E-624.25640364-65
AGCTCCG501.5056429E-424.19927430-31
AGGACCG750.001671886323.7000184
TCGGTCT750.001728304423.56528784-85