FastQCFastQC Report
Tue 15 Mar 2016
59_Embryo23A_140204_L008_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename59_Embryo23A_140204_L008_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166286
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG1900.11426097206018546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCTG350.005044582356.89296786-87
GGCTCTC200.00911297349.0099484-85
ATGCCGC358.305842E-443.483911
CCCCGGA455.6037825E-542.2760243
AAATCGG800.001931384636.60619494
CCCGGAG651.1818862E-535.121624
ATCGGAA950.00448398430.8262794
GGCACAT500.00478282930.4387341
ATGACCC500.00478282930.4387341
CCGGAGA707.453981E-427.2101785
CCTATTC651.8234114E-623.7936816-17
CAGGAGG1352.8962531E-622.5472133
ACCCTAT703.5975409E-622.00334714-15
GGAGGTG900.003174982321.2145778
GTCATCC550.003182615621.21173150-51
GCCAATT900.003242300421.1380123
TTTCCGG600.00336367121.00953160-61
GGTGTGA805.781304E-419.82516764-65
AGGAGGT1207.434459E-419.0931177
TGGTCAT851.0232962E-418.91874348-49