FastQCFastQC Report
Tue 15 Mar 2016
58_Embryo17B_140204_L008_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename58_Embryo17B_140204_L008_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232497
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG5690.24473434065815902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGA1551.4551915E-1146.76315394
ATCGGAA951.9103318E-645.77866494
TAAGGCC357.633629E-444.2430041
CACCACG357.633629E-444.2430042
ACACGTC605.9656995E-638.812818
CACACGT756.6380926E-736.209677
GGGGGTA450.002578295834.5375189
AAATCGG1800.031.89985892-93
ACCTGGG804.349001E-529.034471
GAGCACA804.349001E-529.034474
TCACGGC504.6488167E-628.2282124-25
CCGGGGA550.007007754428.1546364
AAGCCCC550.007007754428.1546361
GCACACG856.4874235E-527.3795626
AGAGCAC856.573641E-527.326563
GTCACGG457.3613184E-526.8840124-25
GCGAATG750.001001114325.8752068
CGAATGC800.00144928424.2841919
CAGGCTT1151.7090679E-724.0645880-81
CTGGGAT1151.8825747E-523.5642073