FastQCFastQC Report
Tue 15 Mar 2016
57_Embryo17A_140204_L008_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename57_Embryo17A_140204_L008_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21009566
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA34150.027.6218471
ATAATCG86600.025.77544894
GCCCCGT25600.025.1844941
GGGCCCT27700.021.7131061
CCCCGAA46800.021.5425452
GGGCCGT22450.021.3940771
CTAGGCG38100.020.48406438-39
GACCCGA15800.020.2656971
CCCCGTA28050.020.0538332
CCCCGTT46850.019.8570982
CCTAGGC40150.019.21708536-37
GCCCCCT34050.019.1887531
CCCGAAT53600.019.1324463
CGTCTGG57550.019.01554986-87
GCCCCGA24350.019.0139241
GGTGTGC66350.018.7266676
GGCCCCT32500.018.5062481
CCCCCGT29900.018.3788971
ACCCGAC33500.018.35390760-61
GGGGGTT54400.017.737521