FastQCFastQC Report
Tue 15 Mar 2016
57_Embryo17A_140204_L008_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename57_Embryo17A_140204_L008_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21009566
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG444500.2115702913615636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGAGG200550.023.0141893
GAGGTGA194900.021.8230489
GCAGGAG209000.021.4981582
AGGAGGT201350.020.6232627
ACCAGGC140700.020.58954878-79
GTGAGCC52450.020.31611894
GTCATCC126800.020.28939250-51
CAGGCTT144700.020.2686180-81
ACCCTAT128550.019.69646814-15
TGCAGGA226150.019.6552581
GGAGGTG223650.019.5446978
CCAGGCT126750.019.4953980-81
TGACCCT133100.018.67489812-13
GGTGACC133900.018.65917410-11
GACCCTA115550.018.54419514-15
TACCACG64250.018.5058989
CTCTACC135050.018.40106422-23
GTGACCC118600.017.8798112-13
CCTATTC141300.017.75414816-17
CTACCGC39550.017.75409394