FastQCFastQC Report
Tue 15 Mar 2016
57_Embryo17A_140204_L008_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename57_Embryo17A_140204_L008_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179240
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAG150.002090790871.081193
CGGTTGA458.0919875E-569.6337388-89
ACCATCG551.431332E-462.04834794
GTCCCAC256.743236E-445.37043866-67
GGCGCGG750.001344804139.4052684-85
GCGCGGC700.001366426139.280780-81
AGCCGGG350.0020331436.24857774-75
ACCTGCT501.5578423E-435.5405961
CGCGGTT450.002753074334.0729686-87
AAGGCGC300.003083286333.30130856-57
ACGCAGG450.003988174731.5916422
CGCGGCG450.00452113630.79261680-81
CGGGCGC450.006190525428.87642376-77
CCAGGCT605.276638E-428.86807480-81
GGTGTGC754.878475E-528.5439787
AAAGGCG350.00717945428.01660754-55
GCGCGGT550.0084200827.11096884-85
TACCAGG757.533643E-526.78107878-79
AAGCCGG751.335166E-424.61995772-73
CGACCAT500.001372185824.5145354-55