FastQCFastQC Report
Tue 15 Mar 2016
56_Embryo11B_140204_L007_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename56_Embryo11B_140204_L007_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186835
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG3780.20231755292102657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCT250.00903645249.11163792-93
GAGCGCG304.6498582E-448.9194229
GCGAGCA503.5181347E-641.9089382-83
GGCGAGC504.4214776E-640.5392980-81
CACACGT400.001933375636.620647
AGGCGAG601.5439475E-533.7827480-81
ACACCGT450.003490292732.464554
AGTGGGG450.003490292732.464551
GCCGCAA450.003490292732.464553
CGAGCAT652.126478E-532.2376482-83
AAATCGG653.174397E-431.48181792-93
TCTCCAG753.1516414E-530.43178288-89
TCGGGAA500.00578315929.2807946
AGTCTGC754.116215E-529.2650935
GGGATGC656.791699E-626.8784658-59
GGGACCA550.00913683326.6618148
GAGCATG658.2556515E-526.42728868-69
GTCTGCC859.6178956E-525.8359936
CGGGATG601.2183089E-424.9585758-59
CCCGCAA451.2663627E-424.820116-17