FastQCFastQC Report
Tue 15 Mar 2016
55_Embryo11A_140204_L007_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename55_Embryo11A_140204_L007_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22421385
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAG127350.045.66197694
TCGGTCC111100.031.66548788-89
GTCGGTC110950.031.35245586-87
CGGTCCT115300.030.5120288-89
AGTCGGT116750.029.69909986-87
TAGTAGC166850.028.9196267
AGGCCGA109250.027.6403336-37
GGGTCAG123100.027.30753380-81
CAGTCGG127700.027.05132784-85
AAGGCCG111250.027.024236-37
GCCCCGT28150.026.9290471
TCAGTCG133200.026.2641984-85
ATGGGTC130200.025.58508178-79
GGTCCTG142150.025.35854790-91
GTCCTGA138400.024.89563690-91
CATGGGT138350.024.24799578-79
GGTCAGT143200.023.73087782-83
CCCCGTT44900.023.443492
GTAGCAA210750.023.1752459
ACATGGG144600.023.1446876-77