FastQCFastQC Report
Tue 15 Mar 2016
55_Embryo11A_140204_L007_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename55_Embryo11A_140204_L007_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236430
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG200.00577927854.9960982-83
CGGTCTA654.6078276E-737.9377676
ACGGTCT708.245861E-735.220475
ACGACGG751.4234975E-632.8446272
CCCTTAT552.8926414E-431.9915222
CAGGCGT450.00416710731.3137136
TCAGGCG450.004171428331.3070855
GCTACTA1000.00417272331.28942186-87
CCAGCCA450.0041887531.2805981
CGACGGT802.3557222E-630.791843
GGACCGA604.7929832E-429.3503915
CAGGAGG1202.1536835E-929.325563
CCAGGCT755.1916322E-528.29016180-81
GGCCAAA500.007010543328.1525361
CAGACGA500.007010543328.1525363
TCGGCCT456.532439E-427.84161656-57
CACGACG905.8941678E-627.3705231
GCGTTAT400.00831002827.19096462-63
ATCGGAA1300.00845961527.07261394
TTCGGCC557.770025E-526.66868854-55