FastQCFastQC Report
Tue 15 Mar 2016
53_Embryo21A_140204_L007_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename53_Embryo21A_140204_L007_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22662186
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG458270.20221791490017776No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG241020.10635337650127838No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA238450.10521932879731903No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA232240.10247908123249892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAG300850.090.8359794
GTCCTGA392350.042.195190-91
TCGGTCC420100.040.2470288-89
GTAGCAA530500.039.967039
TAGTAGC535150.039.448947
CCTGAGA348550.039.19860592-93
AGTCGGT428700.038.780186-87
GTCGGTC426850.038.75881286-87
CGGTCCT441550.038.20477788-89
TCAGTCG451200.037.35733884-85
AGTAGCA567800.037.2860838
CAGTCGG441300.037.08596484-85
GGTCCTG479150.036.26926490-91
GGGTCAG442050.035.8490180-81
GGTCAGT454900.035.56534682-83
ATGGGTC443150.035.2725578-79
GGCCGGC70650.035.011331
CATGGGT449300.034.9383478-79
ACATGGG447300.034.624276-77
TGGGTCA463700.034.38644480-81