FastQCFastQC Report
Tue 15 Mar 2016
52_Embryo8B_140204_L007_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename52_Embryo8B_140204_L007_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21391717
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAG149150.059.49494694
TCGGTCC156300.034.85881488-89
TAGTAGC220600.033.70647
GCCCCGT23000.032.5649571
GTCGGTC160400.032.5083786-87
CGGTCCT164100.032.21486788-89
AGTCGGT174650.030.95244886-87
TCAGTCG176500.030.93605884-85
GTCCTGA170450.030.53162890-91
GGGTCAG171750.029.78217380-81
GGCCGGC42350.029.3049761
GTAGCAA255150.029.2602339
AGGCCGA161600.029.19423736-37
AAGGCCG166750.029.12000836-37
GGTCCTG195550.028.71294290-91
ATGGGTC176900.028.55281878-79
CAGTCGG184250.028.2605384-85
CATGGGT184750.028.19179578-79
GGTCAGT189400.028.14009382-83
GGTATAT167750.027.79206594