FastQCFastQC Report
Tue 15 Mar 2016
52_Embryo8B_140204_L007_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename52_Embryo8B_140204_L007_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226963
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGAGTTGAAGGAAAGACAAAAGCAGCAGCTGAGGCACAAAGCTTTGA2540.11191251437458968No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2270.10001630221666087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC150.00202682871.645880-81
AACGCCT703.6439206E-451.3671594
GTACATC656.858743E-445.19298682-83
GTAGGTA405.0692797E-543.00925478-79
GGTAAGC505.7609097E-542.08191790-91
GACCCCG1051.5303158E-841.0146262-63
CTTGATC357.8039484E-539.97893564-65
CGCAACG654.940084E-737.6010781
GGCAAGT1158.175715E-637.04546780-81
TCCGGTA200.002308525635.33173412-13
GAACCCG1102.3646862E-1134.9152871
TAGGTAC501.8863686E-434.40740678-79
GGCCACT552.6051252E-432.5662780-81
GACTCCC1206.366463E-1132.1260037
CCGTCCG503.2385858E-431.38227872-73
AGTGGGC652.8792874E-530.84328376-77
TAGAGCC703.4471675E-530.0399027
GTCCGTT450.00627183828.80219874-75
CCAACGA500.00703664428.1305529
GGACCCC754.3344698E-728.05275260-61