FastQCFastQC Report
Tue 15 Mar 2016
51_Embryo8A_140204_L007_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename51_Embryo8A_140204_L007_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329802
Sequences flagged as poor quality0
Sequence length10-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC4390.1331101691317821No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG3530.10703391732008903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCT1204.132744E-947.56960380-81
TCCTCGG806.839121E-445.2256194
GTCGGAA806.839121E-445.2256194
AATCGGA1055.013201E-543.07200694
GGACCGT1450.038.615921
GGGGGCG900.00164393437.84278590-91
AAATCGG1201.10134024E-437.68800794
GGACCGA951.8553692E-1036.876695
TCGGCCT554.3166438E-836.495156-57
GCGAGGG900.002246819235.51117386-87
CCGTCTC950.002928998333.64216286-87
GACCGAC1055.366019E-1033.395046
GCGTCCG900.003235923832.9665182-83
CAGCTTA1150.00406535531.46129294
CGAATAC1150.004280431.13191892-93
CGACCCG752.5038098E-630.55925474-75
GACCTCA1301.6552804E-1029.6117273
AATACTC1854.039358E-529.33552794
CGAGTCG1100.029.31077434-35
TACTACC1150.006344316528.72601588-89