FastQCFastQC Report
Tue 15 Mar 2016
50_Embryo6B_140204_L007_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename50_Embryo6B_140204_L007_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22673794
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG333140.14692732940944953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAG218000.078.955894
TAGTAGC350250.039.376057
TCGGTCC270450.038.88501488-89
GTCCTGA269300.038.03138790-91
GTCGGTC275950.036.90159686-87
CGGTCCT285050.036.37875488-89
GTAGCAA382600.036.2272879
AGTCGGT292150.035.5738486-87
TCAGTCG294350.035.2771684-85
CCTGAGA248300.034.60164392-93
GGGTCAG292350.033.83380-81
AGTAGCA409500.033.8052758
GGTCCTG323250.033.51710590-91
ATGGGTC294700.033.11476578-79
GGTCAGT309000.032.94050282-83
CAGTCGG309100.032.8554484-85
CATGGGT305550.032.5418178-79
ACATGGG299150.032.32534876-77
GCCCCGT25400.031.9790291
TGGGTCA318600.031.65709380-81