FastQCFastQC Report
Tue 15 Mar 2016
50_Embryo6B_140204_L007_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename50_Embryo6B_140204_L007_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22673794
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT849050.37446313572399925No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG626190.2761734538119205No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT449240.19813181684547368No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC435430.19204108496354869No Hit
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC383620.16919091705605158No Hit
AAGGCTAAATACAACCACGAGACCGATAGCGAACAAGTACCGTGAGGGAA324590.1431564563036958No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG314540.13872402651272212No Hit
GGACCGTGAAGCGACAATATTTCTCAGGAGTCCCCGAGTCGAACAAGGCT262730.11587385860522505No Hit
GAAAGAGTTGAAGGAAAGACAAAAGCAGCAGCTGAGGCACAAAGCTTTGA246730.1088172539628789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG77700.043.09164494
CAGCTTA128850.039.4213294
GGACCGA198350.039.409995
GACCGAC205250.038.107796
ACCGACT216250.036.1296777
AGGACCG221150.035.3459854
GGACCGT76650.035.1971321
CGACTGA223950.035.1243749
CCGCCCA86850.034.8142594
CCGACTG231750.033.8655248
TCAGGAC237150.033.128022
CAGGACC245500.032.108653
CGCAACG56050.031.2786771
GAACCCG143150.029.4666631
CGGTCTA184000.028.5621136
CCCACCT98250.028.3982942
TCGGCCT146850.028.09067256-57
GGCTAAA170500.027.7808463
CAGGAGG213650.027.7639943
CGGCCTT151500.027.65679756-57