FastQCFastQC Report
Tue 15 Mar 2016
5-Wingbud8A2_131203_L002_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5-Wingbud8A2_131203_L002_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356562
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTA655.7352736E-945.869679
ACAGCGG359.4563E-442.366263
CGGGGGT803.6066922E-837.2428478
GGCGTCC1157.8398443E-1032.239721
ATGCGGG1054.047797E-728.2759025
GTCCGTC1354.3619366E-927.4596124
TGCGGGG1106.026585E-727.0171856
AATGCGG1106.1385254E-726.9603464
CGTCTCG1406.2282197E-926.5571337
GGCACAC858.833282E-526.1710661
GACAGCG750.001308891124.7171172
CGTCCGT1501.3396857E-824.7136523
TCCGTCT1551.875378E-823.94335
GCGGGGG1401.8274659E-723.9014197
CCGGGGA1101.8701508E-523.5903034
GTCTCGT2103.6379788E-1123.0550988
TCTCGTC2154.9112714E-1122.5348079
AAATCGG850.0025574822.0286192-93
TGCGACT850.00271054621.8092212
CGCGGGT603.6879975E-421.1992734-35