FastQCFastQC Report
Tue 15 Mar 2016
49_Embryo6A_140204_L007_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename49_Embryo6A_140204_L007_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28027066
Sequences flagged as poor quality0
Sequence length10-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG320590.11438585829854614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGAG263400.076.0051194
TCGGTCC311350.039.26735788-89
GTCCTGA314000.038.52298790-91
GTCGGTC322050.037.65547686-87
CGGTCCT328250.037.4146388-89
TAGTAGC425400.036.8103687
AGTCGGT340150.035.57237286-87
TCAGTCG351900.035.1863384-85
GGGTCAG337650.034.45504480-81
GTAGCAA457700.034.44539
CAGTCGG355400.033.93123684-85
ATGGGTC342200.033.49289378-79
GGTCCTG375900.033.3762190-91
CCTGAGA300950.033.3008692-93
GGTCAGT356750.032.89002282-83
GCACGCC95900.032.68446494
AGTAGCA481900.032.58198
ACATGGG349050.032.39972376-77
CATGGGT355850.032.10739578-79
TGGGTCA367050.031.55725180-81