FastQCFastQC Report
Tue 15 Mar 2016
49_Embryo6A_140204_L007_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename49_Embryo6A_140204_L007_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28027066
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT804800.287150998966499No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG679190.2423336070925155No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT592430.2113778160011469No Hit
CGATGTTGGATTAAGAAAATTATTAGGTGCAGAAGCTTAATTTATTGGTC547450.19532904371795465No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC452300.1613797177342787No Hit
GGACCGTGAAGCGACAATATTTCTCAGGAGTCCCCGAGTCGAACAAGGCT447030.1594993924801119No Hit
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC389270.1388907422560749No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG325470.11612703234794537No Hit
TGGTAGTAGTAGCAAATACTCTAGCGAGACCCTGGAGGGCTGATGTGGAG309850.11055384819802401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG101950.058.27190894
CTAAGCC100550.040.3109894
GGACCGT124850.037.1576651
CAGCTTA134150.036.97814694
GTGAGCC80450.036.7166394
GGACCGA222050.033.125625
GACCGAC238100.030.9472986
ACCGACT249300.029.5074737
GTCCTCG103450.029.25272892-93
AGGACCG251350.029.2457524
CGGTTAG75600.028.99747794
GACCGTG163000.028.6679382
GAACCCG196250.028.3881741
CGCAACG63450.028.1824321
CCGACTG267400.027.7012168
CGACTGA270150.027.3431079
TCAGGAC271000.027.1718522
CGGTCTA202550.026.9874576
CAGGAGG243600.026.6616923
CCGTGAA179250.026.657234