FastQCFastQC Report
Tue 15 Mar 2016
49_Embryo6A_140204_L007_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename49_Embryo6A_140204_L007_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280858
Sequences flagged as poor quality0
Sequence length10-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4560.16235962657285888No Hit
GGACCGTGAAGCGACAATATTTCTCAGGAGTCCCCGAGTCGAACAAGGCT3770.1342315333727364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA400.001257882980.7515294
TATAAGC200.003802558261.12930780-81
CAGCTTA600.006274468753.83434794
TCCTCGG650.00861031549.69323794
CCGACAT1105.7607394E-849.39417678-79
TAAGCGT354.7442236E-448.7232882-83
TGCCGCG404.776482E-448.6531488-89
GTAACTC600.009620476548.321988-89
AAATCGG953.63867E-745.33418794
ACCATCG956.744795E-445.33418794
AATCGGA1008.6557167E-443.0674894
GCTTTGG1651.0082658E-842.972384-85
CGGACCA652.404704E-838.99045
CCGATTA200.001453536838.8224532-33
GTCGACA550.001906680336.7233490-91
CACCAAG1250.00259938934.45398394
GGACCGA1700.033.543215
AGGACCG1750.032.4920164
GGACCGT1850.032.4432261
GGCACTG1400.031.993879