FastQCFastQC Report
Tue 15 Mar 2016
48-Wingbud6A1_131203_L006_R0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename48-Wingbud6A1_131203_L006_R0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344580
Sequences flagged as poor quality0
Sequence length10-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGT200.00708285452.2452353
ACAGGCG551.3600402E-744.329292
CCGGGGA1550.040.348144
GGCCGGG1650.033.77472
GCCGGGG1550.033.7066043
TCGGTCT603.2384013E-431.3856884-85
GGCCCAT450.004323886731.0822269
GTCGGTC603.669766E-430.72355582-83
CGGGGAA2100.029.8283825
AGCGACC500.00651787928.57923180-81
CGACCTC1103.3252945E-828.49742
CCGGTAG500.00726198427.953598
CGCTATC706.2920255E-527.50928774-75
GAGCCCC508.472807E-426.63209260-61
TGAGCGC1056.8518784E-726.6224658
GAGCGCG1101.0216554E-625.4309129
GCTTCGC851.0704134E-525.35315370-71
GGGTGTG1251.148328E-725.0777133
CTCAGCT1151.5553269E-624.2331986
ACGACCT1301.677854E-724.1131861