FastQCFastQC Report
Tue 15 Mar 2016
47-FW11B1Ovary_131203_L006_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename47-FW11B1Ovary_131203_L006_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28721838
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA26900.031.8959881
CCCCCGT33950.026.402021
GCCCCGT28250.026.2995661
CCCCGAT33400.024.9711232
GGGCCGT24250.024.7077711
CCCCGTA29850.024.2475552
GGGGGGT32100.024.1905271
CCCCGTT51900.023.7356452
GCCCCGA27800.022.414751
GGGGGTT59850.022.2646541
TCCCCGT50300.021.917711
CCCCGAA44950.021.540562
GGGCCCT33400.020.5222451
CCCCGTG26050.019.8724482
GGCCCCT38450.019.696821
CCCGCCG45150.019.20222394
GCGCGCA40700.019.13492290-91
GGGCCGA21050.019.1276911
CGGTACC52250.018.9011755
GCGCACG44750.018.7506492-93