FastQCFastQC Report
Tue 15 Mar 2016
44-FW23B2Ovary_131203_L006_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename44-FW23B2Ovary_131203_L006_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35382796
Sequences flagged as poor quality0
Sequence length10-100
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA41200.027.7386971
CCCCCGT52200.027.0447621
CCACGTA96000.023.3088572
GCACGTC112850.023.14917894
ATAATCG131050.022.89810694
CTACGGG57350.022.64261836-37
CCCCGTG44550.022.4192962
TCCCTCG131600.022.1847231
GGGGGTT83500.021.7376041
GGGGGGT50250.021.500711
GGGCCGT38650.021.493291
GACTACC95350.021.17826338-39
GGACTAC95700.021.05468838-39
ACGGGGC61450.020.83032438-39
GGGGGTG78050.020.7945791
CGGTACC107000.020.7804245
TACGGGG60250.020.76901638-39
ACCTAAG164350.020.7031569
GACCGTC67100.020.67281314-15
CCCGTGG39850.020.47123