FastQCFastQC Report
Tue 15 Mar 2016
43-FW23B2Muscle_131203_L006_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename43-FW23B2Muscle_131203_L006_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22295013
Sequences flagged as poor quality0
Sequence length10-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCAGCCATGGCCTGCACGCGCTTCATCTGGGTTTTCGCCGCCGTGGCCG915440.41060303485806443No Hit
AGGCCGGGGAAACTCATCACCGCTCGCGTGCACGCTCGAGCTACCCGACC825320.37018143923037855No Hit
ATGCCGCAATGGGCCCCTGCTCTCGACTTGACTGCCAACGCGACGCTGGT569440.25541137832034455No Hit
TGCAGGAGGAGGTGACCCTATTCTCTACCAACATTTATTTTGATTCTTTG438430.19664935831165473No Hit
AAGGCTAAATACAACCACGAGACCGATAGCGAACAAGTACCGTGAGGGAA393240.17638025149391032No Hit
CAGCTCAGGCGTGAGTACCCCCTAAATTTAAGCATATTAATAAGGGGAGG381420.17107861744687028No Hit
TGAAACCCAAAGAATTTGGCGGTGTTTTAGTCCTTTCAGAGGAATCTGTC361850.16230086970570504No Hit
AACACGGACCAAGGAGTCTAACATGTGCGCGAGTCAATGGGAGCTTCTAA331850.1488449457284461No Hit
CAGCCACAAGCACGTCCCCTAGAGTTTCAGCTAGTGAACAAACGCGATGT301880.1354024776751644No Hit
CGTACCACCTCGGAGGCAAAATCGAATCCAACCTCCAGTCCATTCAACCT275950.12377207405082023No Hit
CGACGATCAGGAACTGGCCAGCAACGCTCAGACTGCTCTTCATCTCCTCA261970.11750161347741757No Hit
ACGACCTCAGCTCAGGCGTGAGTACCCCCTAAATTTAAGCATATTAATAA254790.11428116233886025No Hit
CAACAACCCCGACGACGAGGAGCTAGCCAGCAACGCTCAGGCCGCGCTGC228120.10231884592307705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCT120650.081.3212294
TTCAGCC166100.054.9407771
GCAGAGC198550.048.9800494
ATGGCCT188150.048.9240468
CCGCAAT128400.047.8109444
CCATGGC193600.047.475486
CAGCCAT195150.047.299953
ATGGGCC145550.041.3941429
CAGCTTA116750.040.11901594
CCGGGGA215400.039.8344574
TCAGCCA236600.039.504812
GCCGGGG220850.038.5714263
AGCCATG241950.038.4612164
ATCGCGG122850.038.3012164-65
TGGCCTG244800.038.037749
GCGCACA121100.037.9552584-85
GCCATGG247550.037.8064885
CGGACCA94000.037.642265
GGCCGGG230900.037.5489122
ATGCCGC163000.037.2875941