FastQCFastQC Report
Tue 15 Mar 2016
41-FW17B3Ovary_131203_L006_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41-FW17B3Ovary_131203_L006_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29544754
Sequences flagged as poor quality0
Sequence length10-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGA28550.029.6522871
GCCCCGT32400.025.538311
CCCCGTA30000.022.9579072
CCCCGAT33500.022.2725962
GGGGGTT68950.021.7120631
GGGGGGT35800.021.6432781
GCCCGGT47900.021.3682672
GGGCCGT24750.020.8708251
CCCGGTA58550.020.5719813
GGGCCCT35650.020.526881
GGGGGTA52000.020.511271
CCCCGTT57300.020.3669622
CCCGCCG48300.020.23695494
CCCCCGT31500.019.738941
GGGGCTA33700.019.7276761
CCCCGAA46600.019.603722
CGGGACG41150.019.5756829
GGGGGTG61300.019.5061051
GCCCCGA27850.019.234631
CGGTACC66500.019.2272225